Does it go between the carb and spacer or between the spacer and manifold?
Depends on what other ports and such, but I would guess between the spacer and the carb. If your spacer has exhaust gasses routed through it underneath, those would shred that gasket pretty quickly i would think.
If you don't have any additional exhaust gas circuits though, it could probably go in either position.
I'm sure the others will know for sure though. Been a long time since I had one.
Do you still run the stock aluminum spacer with the PCV connection?
Yes, absolutely. You want to keep the PCV fully functional whenever you can.
Are you sure it's aluminum though? If so I would inspect it carefully for damage/wear/tear to make sure it will still seal.
The aluminum ones took a beating wherever the exhaust gasses were routed through them. Depending on the year of your Bronco you may have had EGR ('73 and later) or even just a heat riser circuit cut into it.
And they get caked up pretty good sometimes from the PCV.
I may be thinking of other engines with the exhaust gasses, but it's still worth taking a look at when it's apart.
Clean it if that's all it needs, replace it if it's buggered.
If it's already cast-iron or steel though, you are probably good to go.
Paul