Yes, normally anyway.
The new wire that powers the fuse box (probably a big Red one?) usually marked as such, will need a fuse or fusible link to protect the main circuit.
The factory used a fusible link, but I like replaceable fuses myself. Never had one blow, but if it did out on the trail, the fix would be much easier with a fuse. Assuming you could find the actual offending short first of course.
I like 60 amp Maxi-Fuses myself. Bigger than most push-in fuses, but not as bulky as the Mega-Fuse we typically use in the charging circuit.
paul