If you mean the all-Black and Black w/yellow wires, then as was said they are both positive.
AND YOU DO NOT USE EITHER OF THOSE WIRES FOR A VOLT-METER.
Sounds like Painless still leaves it up to the installer to connect the two wires together with a supplied crimp connector? If so you are basically going to connect them as expected, but separate from the gauges because there is no longer an ammeter able to use them.
You do NOT want to use that charge wire for connecting to a volt-meter or you will have really big problems you don't want to know about. In other words you "let the smoke out" of the wires.
Sorry if you knew that part already, but it's important enough to repeat.
So to reiterate, for the wire that Painless says is for the ammeter, you just leave it alone and in one piece, just tied up out of the way under the dash.
Any of the extra smaller "switched" wires in the new harness can be used for the one terminal on the gauge (whichever one everyone confirms is correct) and the other should be a ground. That ground you likely have to make up yourself.
What alternator are you using by the way? This is just for lower amp stock type alternators anyway.
Paul